Transformation of Yeast
***All resources are cited in MLA format, links for articles are provided.
[1] "Yeast Transformation Protocols." Sigma-Aldrich. N.p., n.d. Web. 23 Nov. 2015.
http://www.sigmaaldrich.com/technical-documents/protocols/biology/yeast-transformation-protocols.html#sthash.mzni23wL.dpuf
[2] Gietz, R. D., and R. A. Woods. Review. Place of Publication Not Identified: Publisher Not Identified, 1863. Biotechniques, Apr. 2001. Web.
http://www.biotechniques.com/multimedia/archive/00010/01304rv02_10885a.pdf
[3] Kawai, Shigeyuki, Wataru Hashimoto, and Kousaku Murata. "Transformation of Saccharomyces Cerevisiae and Other Fungi."Bioengineered Bugs 1.6 (2010): 395-403. Web.
http://www.tandfonline.com/doi/pdf/10.4161/bbug.1.6.13257
[4] Transformation of saccharomyces cerevisiae by electroporation. Appl Environ Microbiol. 1989
http://aem.asm.org/content/55/9/2242.short
[5] Costanzo MC, Fox TD. Transformation of Yeast by Agitation with Glass Beads.Genetics. 1988;120(3):667-670
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1203545/
[6] Hinnen A, Hicks JB, Fink GR. Transformation of yeast. Proc Natl Acad Sci USA 1978; 75:1929-33
http://www.ncbi.nlm.nih.gov/pubmed/347451
[7] Ito H, Fukuda Y, Murata K, Kimura A. Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology. 1983;153(1):163-168.
http://jb.asm.org/content/153/1/163.short?rss=1&ssource=mfc
[8] Hashimoto H, Morikawa H, Yamada K, Kimura A. A novel method for transformation of intact yeast cells by electroinjection of plasmid
http://link.springer.com.prox.lib.ncsu.edu/article/10.1007%2FBF00252714
[9] Yamakawa M, Hishinuma F, Gunge N. Intact cell transformation of Saccharomyces cerevisiae by polyethylene glycol. Agric Biol Chem 1985; 49:869-71
https://www.jstage.jst.go.jp/article/bbb1961/49/3/49_3_869/_article
[10] Burgers PM, Percival KJ. Transformation of yeast spheroplasts without cell fusion. Anal Biochem 1987; 163:391-7
http://www.sciencedirect.com.prox.lib.ncsu.edu/science/article/pii/0003269787902405
[11] Constanzo MC, Fox TD. Transformation of yeast by agitation with glass beads. Genetics 1988; 120:667-70.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1203545/?tool=pmcentrez
[12] Johnston SA, Anziano PQ, Shark K, Sanford JC, Butow RA. Mitochondrial transformation in yeast by bombardment with microprojectiles. Science 1988; 240:1538-41
[13] Delorme E. Transformation of Saccharomyces cerevisiae by electroporation. Appl Environ Microbiol 1989; 55:2242-6
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC203062/?tool=pmcentrez
[14] Schiestl RH, Gietz RD. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr Genet 1989; 16:339-46
http://link.springer.com.prox.lib.ncsu.edu/article/10.1007%2FBF00340712
[15] Armaleo D, Ye GN, Klein TM, Shark KB, Sanford JC, Johnston SA. Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi. Curr Genet 1990; 17:97-103.
http://link.springer.com.prox.lib.ncsu.edu/article/10.1007%2FBF00312852
[16] Chen DC, Yang BC, Kuo TT. One-step transformation of yeast in stationary phase. Curr Genet 1992; 21:83-4
http://link.springer.com.prox.lib.ncsu.edu/article/10.1007%2FBF00318659
[17] Gietz D, St. Jean A, Woods RA, Schiestl RH. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 1992; 20:1425
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC312198/?tool=pmcentrez
[18] Gietz RD, Schiestl RH, Willems AR, Woods RA. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 1995; 11:355-60
[19] Thompson JR, Register E, Curotto J, Kurtz M, Kelly R. An improved protocol for the preparation of yeast cells for transformation by electroporation. Yeast 1998; 14:565-71
[20] Hayama Y, Fukuda Y, Kawai S, Hashimoto W, Murata K. Extremely simple, rapid and highly efficient transformation method for the yeast Saccharomyces cerevisiae using glutathione and early log phase cells. J Biosci Bioeng 2002; 94:166-71
http://www.sciencedirect.com.prox.lib.ncsu.edu/science/article/pii/S1389172302801384
[21] Gietz RD, Woods RA. Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol 2002; 350:87-96
http://www.sciencedirect.com.prox.lib.ncsu.edu/science/article/pii/S0076687902509575
[22] Gietz RD, Schiestl RH. Frozen competent yeast cells that can be transformed with high efficiency using the LiAc/ SS carrier DNA/PEG method. Nat Protoc 2007; 2:1-4
http://search.proquest.com.prox.lib.ncsu.edu/docview/1041220613?pq-origsite=summon
[23] Mell, Joshua Chang, and Sean M. Burgess. "Yeast as a Model Genetic Organism." Encyclopedia of Life Sciences (2001): n. pag. Web.
http://www.marchelli.it/Uni/OrganismiModello/Yeast/Yeast%20as%20Model%20Genetic%20Organism.pdf
[25]Kawai S, Hashimoto W, Murata K. Transformation of Saccharomyces cerevisiae and other fungi: Methods and possible underlying mechanism.Bioengineered Bugs. 2010;1(6):395-403. doi:10.4161/bbug.1.6.13257.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3056089/
[26]Rejman J, Bragonzi A, Conese M. Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes. Mol Ther. 2005;12:468–474
http://www.ncbi.nlm.nih.gov/pubmed/15963763
[27] Goller, Carlos. "Tools for Expression and Metabolic Engineering." NC State BIT495. Nov. 2015. Lecture.
[29] https://www.youtube.com/watch?v=7UwVJX8nwTA
[30] "Stanford Researchers Genetically Engineer Yeast to Produce Opioids."Stanford University. N.p., n.d. Web. 23 Nov. 2015.
http://news.stanford.edu/news/2015/august/opioids-yeast-smolke-081315.html
[31] Ledford, Heidi. "Sirtuin Protein Linked to Longevity in Mammals."Nature.com. Nature Publishing Group, 22 Feb. 2012. Web. 23 Nov. 2015.
http://www.nature.com/news/sirtuin-protein-linked-to-longevity-in-mammals-1.10074
[32] Methods in Molecular Biology, Vol 53 Yeast Protocol, pg 147-151.
http://www.springerprotocols.com/BookToc/doi/10.1385/0896033198
Prezi Resources
Mousdale DM. Biofuels: Biotechnology, Chemistry, and Sustainable Development. CRC Press: Taylor & Francis Group, 2008. Print.
Kuyper, M. et al. Minimal Metabolic Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Xylose Fermentation: A Proof of Principle. FEMS Yeast Research, 2004. 4(6):655-664.
Matuo, R. et al. Saccharomyces cerevisiae as a Model System to Study the Response to Anticancer Agents. Cancer Chemother Pharmacol, 2012. Oct;70(4):491-502.
Picture Resouces
Website Background: http://static.guim.co.uk/sys-images/Guardian/Pix/pictures/2011/9/14/1316029994927/Brewers-yeast-007.jpg
Figure.1 http://nptel.ac.in/courses/102103047/module7/lec38/images/5.png
Figure.2 https://www.btxonline.com/ecm-630-exponential-decay-wave-electroporation-system/
Figure.3 &Figure.4 Borodina, Irina, and Jens Nielsen. "Advances in Metabolic Engineering of Yeast Saccharomyces Cerevisiae for Production of Chemicals."Biotechnology Journal 9.5 (2014): 609-20. Web.
http://onlinelibrary.wiley.com.prox.lib.ncsu.edu/doi/10.1002/biot.201300445/full
Figure.5 Abate, Tom. "Stanford Researchers Genetically Engineer Yeast to Produce Opioids." Stanford University. Stanford News, 13 Aug. 2015. Web. 23 Nov. 2015.
http://news.stanford.edu/news/2015/august/opioids-yeast-smolke-081315.html
Figure.6 "Bench Philosophy: Yeast Recombinational Cloning." Labtimes:. Lab Times Online, Mar. 2010. Web. 23 Nov. 2015.
http://www.labtimes.org/labtimes/method/methods/2010_03.lasso