METHODS OF SELECTION

 

Metabolic selection:

Yeast transformants are usually selected using auxotrophic markers and the appropriate synthetic medium is used for screening transformants. The vector DNA provides a gene required for metabolism like URA3 (uracil biosynthesis), LEU2, TRP1 or HIS3. URA3 is the most common one. The plasmid DNA has a normal copy of the yeast URA3 gene, as well as the URA3 promoter and the yeast mutant strain lacks the URA3 allele. Therefore, transformed yeast cells are the only cells containing a functional copy of the URA3 gene, which allows them to grow on on a medium without Uracil.

List of auxotrophic markers: http://wiki.yeastgenome.org/index.php/Commonly_used_auxotrophic_markers

See more at: http://www.sigmaaldrich.com/technical-documents/protocols/biology/yeast-transformation-protocols.html#sthash.488tzC9t.dpuf

 

Antibiotic selection:

The antibiotic in yeast are different from the antibiotic used in bacteria. DNA provides resistance to eukaryotic antibiotic such as G418, hygromycin or Zeocin. Vectors containing the Kan4MX gene enable cells to grow in the presence of the eukaryotic antibiotic G418 or geneticin.

 

Find out more about how to select which method to use: